Sperm mitochondrial superoxide anion(O₂^-.) detection

Sperm Reactive Oxygen Species MitoSOX Red Staining kit

Phyiological& Pathological role of sperm mitochondria:

The mitochondria are the main site of ROS production in sperm. ROS play an important role in sperm physiology acting as triggers of fertilization processes such as hyperactivation, acrosome reaction and spermatozoa–oocyte binding. However, in cases of mitochondrial dysfunctions, there is an imbalance between ROS production and antioxidant capacity, the oxidative stress. In this case, ROS cause damage to sperm structures including lipid peroxidation of the plasma membrane and DNA damage leading to loss of biological function of spermatozoa.

Features:

• Specific targeting probe for mitochondria, with an accurate result.
• Exclude the interference of dead sperm, and report the mitochrondrial ROS level in live sperm, which is more clinically meaningful.
• A large number of sperm can be easily analyzed, leading to good repeatable results.
• Required device: flow cytometer (blueexcitation).

Apllications:

• Analyze the etiology of infertility.

• Analyze the reasons of ART adverse outcomes(low fertilization and blastocyst formation rate, miscarriage).

• Analyze the reasons of low quality semen parameters.

Test results:

Q1: dead sperm, mitochondria O₂^-. (-); Q2: dead sperm, mitochondria O₂^-. (+)

Q3: live sperm, mitochondria O₂^-. (-); Q4: live sperm, mitochondria O₂^-. (+)

BRED Life Science Technology Inc. specializes in developing, manufacturing and marketing lab products in the field of reproductive medicine, offering automatic assay platform and related products for infertility diagnosis and ART treatment. Based on the test methods and standard recommended by the World Health Organization, BRED develops serial products for infertility diagnosis and ART treatment with innovative technology. And these help promote the establishment and popularization of standardization for reproductive medicine lab. BRED own a completest range of relative products in the field of reproductive medicine worldwide.The products are of independent intellectual property right within BRED.

Our mission of development is to be devoted to providing the best solutions and products for the clinical laboratory in reproductive medicine with the aim to promote the development of human reproductive health.

Our operating philosophy is putting people first, doing business on the basis of honesty, forging ahead and working with diligence.

Welcome to visit our official website: www.spermfunc.com