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40T/Kit Sperm Function Test Kit For Determinate Protein Tyrosine Phosphorylation

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40T/Kit Sperm Function Test Kit For Determinate Protein Tyrosine Phosphorylation

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Brand Name :	BRED
Model Number :	BRED-024
Certification :	CE, ISO13485
Price :	Negotiable
Payment Terms :	T/T
Supply Ability :	10000 kits per year
Delivery Time :	3 - 5 working days

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40T/Kit Sperm Function Test Kit For Determinate Protein Tyrosine Phosphorylation

Male Infertility Diagnosis Sperm Function Kit for determination of Protein Tyrosine Phosphorylation of Capacitated Sperm

The kit is mainly used for detecting the protein tyrosine phosphorylation lever in mammalian sperm capacitation.

Fluorescent Staining. A series of complicated biochemical and molecular response of enhance fluidity of serosa, hyper activated movement and cAMP activate after sperm capacitation. Which made sperm tail proceed protein tyrosine phosphorylation. In which the sperm can combine with antibody of Anti-Tyrosine Phosphorylation signed by fluoresceine isothiocyanate (FITC). Green fluorescence can be detected in sperm tail at excitation wavelength between 450nm – 490nm.

Features:

This is an alternative test for Sperm - zona pellucida binding test, which can reflect the binding ability of the sperm with zona pellucida.
Help to choose the assisted reproductive way scientifically
Double marking technology, easy to observe results
40T/Kit
CE approved

Storage Condition:

Dedicated tissue slides, Long shaped coverslip and glass slide barrel can keep at room temperature.
Divide the unseal Krebs-Ringer Medium of sperm into several parts and store at -20°C, freeze-thawing repeatedly is not allow.
Other reagent need to keep in dark place (2-8°C).

Period of validity:
For professional use only 1/6

The kit store at 2-8°C ,period of validity is 2 years.
The reagent store at -20°C, the period of validity for using is the same as the kit`s.
Once unsealed, the kit would be kept for 45 days at 2-8° C.

Applicable instruments:

Fluorescence microscope

Other equipment:

Refrigerator (2-8°C)
Freezer (-20°C)
Water bath (37°C)
Low velocity centrifugal machine
Optical microscope
Carbon dioxide incubator
Bechtop
Counting device of sperm

Other materials required but not provided

Semen sampling device
Liquefier
Digital adjustable pipette and disposable tips (1-10μl)
Disposable plastic tubularis
Glass tube (5ml)
Cone-shape centrifuge tube (15ml)
Eppendorf tube
Wet box

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